CRISPR Screen Dataset
Leng K (2022) - 6-PMID36303069
Title: CRISPRi screens in human iPSC-derived astrocytes elucidate regulators of distinct inflammatory reactive states.
Screen Details:
- Screen Rationale : Regulation of inflammatory reactivity
- Cell Type : HiPSC-derived Astrocyte
- Cell Line : iAstrocyte
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : IL-1α+TNF+C1q
- Library : CRISPRi Targeted Transcription Factor Library (Kampmann, 2022) | Type: CRISPRi | Format: Pool | Enzyme: dCas9-BFP-KRAB | Methodology: Inhibition
- Analysis Method : MAGeCK-iNC | Number of Hits: 29 | Full Dataset Size: 3,598
- Experimental Setup : Cytokine exposure | Duration: 24 Hours
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative gene score are enriched in a population with reduced inflammatory reactivity marker VCAM1 upon KO in the presence of IL-1a+TNF+C1q, suggesting the gene normally enhances VCAM1 levels in the presence of cytokines
- Genes with a significant positive gene score are enriched in a population with increased inflammatory reactivity marker VCAM1 upon KO in the presence of IL-1a+TNF+C1q, suggesting the gene normally inhibits VCAM1 levels in the presence of cytokines
Score Distribution
< 9.476852405
> -5.892973854