CRISPR Screen Dataset
Tian M (2023) - 12-PMID36584827
Title: Human functional genomics reveals toxicological mechanism underlying genotoxicants-induced inflammatory responses under low doses exposure.
Screen Details:
- Screen Rationale : Decreased drug resistance
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
- Phenotype : Response To Chemicals
- Condition : 4-Nitroquinoline N-oxide (NQO) [PubChem]
- Library : GECKO | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RIGER | Number of Hits: 228 | Full Dataset Size: 18,080
- Experimental Setup : Drug Exposure | Duration: 10 Days | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of hit genes results in increased sensitivity to NQO
Score Distribution
< 0.1055
> 0.000274