CRISPR Screen Dataset
Prolo LM (2019) - 1-PMID31570734
Title: Targeted genomic CRISPR-Cas9 screen identifies MAP4K4 as essential for glioblastoma invasion.
Screen Details:
- Screen Rationale : Essential for glioblastoma invasion
- Cell Type : Glioblastoma Cell Line [BTO:0001530]
- Cell Line : U-138MG [BTO:0002127, CELLOSAURUS:CVCL_0020]
- Phenotype : Cell Migration [CMPO:0000033]
- Condition : Cells grown in a transwell with matrigel and allowed to invade toward a higher concentration of serum in the bottom chamber
- Library : Human CRISPR Deletion Library - Drug targets, kinases, phosphatases and Human CRISPR Deletion Library - Trafficking, mitochondrial, motility [AddGene, AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 52 | Full Dataset Size: 4,574
- Experimental Setup : Other | Duration: 36 Hours | MOI: = 0.2
- Significance : Authors have indicated that results with Score.4 (FDR) = 1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Cells were grown in a transwell with matrigel and invading vs non-invading populations were separated.
- Hit genes were those whose KO led to significant enrichment in the non-migrating cell population compared to the migrating population, suggesting the gene is essential for glioblastoma invasion.
Score Distribution
< 108
> 0