CRISPR Screen Dataset
Strezoska Ž (2017) - 1-PMID28445693
Title: High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries.
Screen Details:
- Screen Rationale : Cell cycle regulators
- Cell Type : Osteosarcoma Cell Line [BTO:0000407]
- Cell Line : U2OS [BTO:0001938, CELLOSAURUS:CVCL_0042]
- Phenotype : Cell Cycle Progression [APO:0000253]
- Library : Edit-R crRNA Cell Cycle Regulation library | Type: CRISPRn | Format: Array | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Reduced Chi Squared | Number of Hits: 41 | Full Dataset Size: 169
- Experimental Setup : Timecourse | Duration: 72 Hours
- Significance : Authors have indicated that results with Score.1 (Reduced Chi Squared) > 6.0 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- U2OS cells expressing a G1S Cell Cycle Phase Marker (G1S-CCPM) were utilized to evaluate cell cycle phenotypes based on multiple parameters including nuclear size and morphology and reporter localization
Score Distribution
< 502.1
> 0.7