CRISPR Screen Dataset
Strezoska Ž (2017) - 1-PMID28445693
Title: High-content analysis screening for cell cycle regulators using arrayed synthetic crRNA libraries.
Screen Details:
- Screen Type : Phenotype Screen | Format: Array
- Cell Line : U2-OS cell | Type: Osteosarcoma Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Cell Cycle Progression
- Experimental Setup : Timecourse | Duration: 72 Hours
- Library : Edit-R crRNA Cell Cycle Regulation library | Type: CRISPRn | Methodology: Knockout
- Analysis Method : Reduced Chi Squared | Number of Hits: 41 | Full Dataset Size: 169
- Significance : Authors have indicated that results with Score.1 (Reduced Chi Squared) > 6 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- U2OS cells expressing a G1S Cell Cycle Phase Marker (G1S-CCPM) were utilized to evaluate cell cycle phenotypes based on multiple parameters including nuclear size and morphology and reporter localization
Score Distribution
< 502.1
> 0.7