CRISPR Screen Dataset
Lau MT (2019) - 1-PMID31040274
Title: Molecular dissection of box jellyfish venom cytotoxicity highlights an effective venom antidote.
Screen Details:
- Screen Rationale : Increased resistance to toxin
- Cell Type : Chronic Myelogenous Leukemia Cell Line []
- Cell Line : HAP-1 [EFO:0007598, CELLOSAURUS:CVCL_Y019]
- Phenotype : Response To Toxin
- Condition : CfTX-1 (Chironex fleckeri toxin) (1 µg/mL) [Uniprot]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,012 | Full Dataset Size: 20,915
- Experimental Setup : Toxin Exposure | Duration: 14 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hits represent genes whose KO led to increased growth in the presence of venom; normal function of the gene is required for jellyfish venom killing.
Score Distribution
< 1
> 1.55e-17