CRISPR Screen Dataset
Kramer NJ (2018) - 1-PMID29507424
Title: CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity.
Screen Details:
- Screen Rationale : Response to dipeptide-repeat proteins
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Condition : glycine–arginine (GR) dipeptide-repeat (DPR) protein (10.0 µM)
- Library : Bassik Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 387 | Full Dataset Size: 20,631
- Experimental Setup : Toxin Exposure | Duration: 3 Weeks | MOI: < 1.0
- Significance : Authors have indicated that results with Score.1 (CasTLE Score) > 19.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of hit genes has either a protective (positive effect score) or sensitizing (negative effect score) effect on cells in the presence of glycine-arginine (GR) dipeptide repeats derived from C9ORF72 expansion
Score Distribution
< 517
> 0