CRISPR Screen Dataset
Russo M (2020) - 1-PMID32421776
Title: Functional Pathway Identification With CRISPR/Cas9 Genome-wide Gene Disruption in Human Dopaminergic Neuronal Cells Following Chronic Treatment With Dieldrin.
Screen Details:
- Screen Rationale : Increased and decreased resistance to toxin
- Cell Type : Neuroblastoma Cell Line [BTO:0000932, EFO:0005214]
- Cell Line : SH-SY5Y [BTO:0000793, CELLOSAURUS:CVCL_0019]
- Phenotype : Response To Toxin
- Condition : Dieldrin (12.5 µM) [ChEBI]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 99 | Full Dataset Size: 19,114
- Experimental Setup : Toxin Exposure | Duration: 30 Days | MOI: = 0.35
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.0 AND Score.2 (FDR) < 0.1 OR Score.1 (Log2FC) < 0.0 AND Score.2 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- A CRISPR knockout library was used to screen human neuronal cells for genes whose loss confers increased or decreased resistance to Dieldrin. This ORCS record contains only the 100 gene hits identified by the gene-based MaGeCK analysis.
Score Distribution
< 1.8208
> -3.4054