CRISPR Screen Dataset
MacLeod G (2019) - 13-PMID30995489
Title: Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells.
Screen Details:
- Screen Rationale : Increased drug sensitivity
- Cell Type : Glioblastoma Cell Line [BTO:0001530]
- Cell Line : G432NS-Cas9 [CELLOSAURUS:CVCL_C9AI]
- Phenotype : Response To Chemicals
- Condition : Temozolomide (20 LD (%Lethal Dose)) [ChEBI]
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Delta Z scores | Number of Hits: 173 | Full Dataset Size: 16,935
- Experimental Setup : Drug Exposure | Duration: 10 Doublings | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Z-score) >= 2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Phenotypic readout: cell proliferation
- the authors compared Bayes Factor scores from TKOv1 infected glioblastoma cells screened in the presence and absence of sub-lethal doses of TMZ to determine genes that sensitize cells to the drug
Score Distribution
< 4.47
> -4.745