CRISPR Screen Dataset
Yu JSL (2019) - 1-PMID30932271
Title: CRISPR-Knockout Screen Identifies Dmap1 as a Regulator of Chemically Induced Reprogramming and Differentiation of Cardiac Progenitors.
Screen Details:
- Screen Rationale : Regulators of reprogramming and differentiation
- Cell Type : Mouse Cell [CLO:0007836]
- Cell Line : primary cultured fibroblast [CLO:0037301]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Reprogramming conditions (5% O2, 2 μM SB431542/Alk5i)
- Library : Mouse improved genome-wide library v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 14 | Full Dataset Size: 18,424
- Experimental Setup : Timecourse | Duration: 17 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Depletion-Enrichment (DE) score) > 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened Cas9 expressing primary cultured mouse cardiac fibroblasts for genes effecting the reprogramming of differentiated fibroblasts into multi-potent progenitor cells using the progenitor cell marker Nkx2-5 as a reporter
- Authors used a MaGeCK derived p-values to generate a depletion-enrichment (DE) score for each gene. A positive DE score indicates that loss of that particular gene is advantageous for reprogramming.
Score Distribution
< 4.122025697
> -4.486495284