CRISPR Screen Dataset
MacLeod G (2019) - 5-PMID30995489
Title: Genome-Wide CRISPR-Cas9 Screens Expose Genetic Vulnerabilities and Mechanisms of Temozolomide Sensitivity in Glioblastoma Stem Cells.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Glioblastoma Cell Line [BTO:0001530]
- Cell Line : G477NS-Cas9 [CELLOSAURUS:CVCL_C9A2]
- Phenotype : Cell Proliferation
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,931 | Full Dataset Size: 16,975
- Experimental Setup : Timecourse | Duration: 14 Doublings | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 3.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes have a minimum BF of 3
Score Distribution
< 113.714
> -65.727