CRISPR Screen Dataset
Xia T (2023) - 1-PMID36920967
Title: Glucosylceramide is essential for Heartland and Dabie bandavirus glycoprotein-induced membrane fusion.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Response To Virus
- Condition : Virus: Heartland virus (HRTV) (Strain: MO4) (1.0 MOI) [NCBI Taxid 1216928]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 807 | Full Dataset Size: 21,669
- Experimental Setup : Virus Exposure | Duration: 15 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.2 (p-Value) < 0.05 AND Score.4 (Log2FC) > 2.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Hit genes show sgRNA enrichment in the presence of HRTV, suggesting that KO of the gene protects cells from infection and the gene normally plays a role in viral entry and infection
Score Distribution
< 0.99998
> 0.0000224