CRISPR Screen Dataset

Xu P (2021) - 1-PMID33156908

H. sapiens

Title: FBXO11-mediated proteolysis of BAHD1 relieves PRC2-dependent transcriptional repression in erythropoiesis.

Screen Rationale : Regulation of erythroid maturation
Cell Type : Umbilical Cord Erythroid Progenitor
Cell Line : HUDEP-2 [CELLOSAURUS:CVCL_VI06]
Phenotype : Protein/peptide Accumulation [APO:0000149]
Library : CRISPRn UPS targeted library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MaGeCK | Number of Hits: 46 | Full Dataset Size: 773
Experimental Setup : Timecourse | Duration: 5 Days | MOI: ~ 0.3
Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by 'Yes' in the HIT column of the table below.

Hit genes with a negative log-fold change are under-represented in mature Band3+ vs immature Band3- erythroblasts upon KO, suggesting the gene normally acts as a positive regulator of erythroid maturation
Hit genes with a positive log-fold change are over-represented in mature Band3+ vs immature Band3- erythroblasts upon KO, suggesting the gene normally acts as a negative regulator of erythroid maturation

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