CRISPR Screen Dataset

Marschallinger J (2020) - 1-PMID31959936

M. musculus

Title: Lipid-droplet-accumulating microglia represent a dysfunctional and proinflammatory state in the aging brain.

Screen Rationale : Regulators of lipid droplet formation
Cell Type : Microglial Cell Line [BTO:0003349]
Cell Line : BV-2 [BTO:0003350, CELLOSAURUS:CVCL_0182]
Phenotype : Regulation Of Lipid Localization [GO:1905952]
Condition : 5 μg/ml LPS in DMEM with 5% FBS
Library : CRISPRn targeted library (Wyss-Coray, 2020) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : CasTLE | Number of Hits: 219 | Full Dataset Size: 2,807
Experimental Setup : Timecourse | Duration: 90 Hours | MOI: < 1.0
Significance : Authors have indicated that results with Score.3 (p-Value) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Positive casTLE effect size represents genes targeted by sgRNAs that were enriched in lipid droplet- negative cells; negative effect size represents genes targeted by sgRNAs that were under-represented in lipid droplet- negative cells.

< 34.8

> 0