CRISPR Screen Dataset
Yang X (2020) - 1-PMID32924251
Title: PEBP1 suppresses HIV transcription and induces latency by inactivating MAPK/NF-κB signaling.
Screen Details:
- Screen Rationale : HIV-1 latency promoting genes
- Cell Type : T-lymphoblastic Leukemia Cell Line [BTO:0002504]
- Cell Line : C11 [CELLOSAURUS:CVCL_C8ZJ]
- Phenotype : Response To Virus
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 1,156 | Full Dataset Size: 19,050
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: = 0.2
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- KO of hit genes results in increased expression of HIV-1 proviral DNA encoding a GFP reporter. The reporter is silenced in the latent state, loss of the gene causes increased GFP expression indicating loss of the latent state
Score Distribution
< 38304
> 2