CRISPR Screen Dataset
Takahashi Y (2019) - 1-PMID31519728
Title: VPS37A directs ESCRT recruitment for phagophore closure.
Screen Details:
- Screen Rationale : Autophagosome completion genes
- Cell Type : Osteosarcoma Cell Line [BTO:0000407]
- Cell Line : U2OS [BTO:0001938, CELLOSAURUS:CVCL_0042]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : Brunello (human) [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 177 | Full Dataset Size: 19,114
- Experimental Setup : Timecourse | Duration: 3 Hours
- Significance : Authors have indicated that results with Score.1 (p-Value) < -1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors used HT-LC3 expressing cells to measure the accumulation of phagophores due to defects in autophagosome completion
- Cells with high HT-LC3 expression represent genes whose KO leads to defects in autophagosome completion
Score Distribution
< 1
> -2.358