CRISPR Screen Dataset
Fei T (2017) - 1-PMID28611215
Title: Genome-wide CRISPR screen identifies HNRNPL as a prostate cancer dependency regulating RNA splicing.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Prostate Cancer Cell Line [BTO:0001033]
- Cell Line : LNCaP [BTO:0001321, CELLOSAURUS:CVCL_0395]
- Phenotype : Cell Proliferation
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 1,991 | Full Dataset Size: 21,812
- Experimental Setup : Timecourse | Duration: 14 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) <= 0.008592 OR Score.3 (MaGeCK Score) <= 0.0099839 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a significant negative MAGeCK score are cell-essential genes in the LNCaP cell line, their KO results in decreased proliferation
- Genes with a significant positive MAGeCK score are growth restrictive genes in the LNCaP cell line, their KO results in increased proliferation
Score Distribution
< 0.99998
> 0.00000167