CRISPR Screen Dataset
Wang T (2017) - 18-PMID28162770
Title: Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Pre-B-Lymphocyte Cell Line [BTO:0000743]
- Cell Line : BA/F3 [BTO:0001516]
- Phenotype : Cell Proliferation
- Condition : Murine Interleukin 3 (1 ng/mL) [Uniprot]
- Library : Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 3,296 | Full Dataset Size: 18,987
- Experimental Setup : Timecourse | Duration: 14 Doublings
- Significance : Authors have indicated that results with Score.1 (Log2) < -1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Score Distribution
< 1.966
> -7.324