CRISPR Screen Dataset
Wang T (2017) - 8-PMID28162770
Title: Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : OCI-AML-2 [BTO:0004619, CELLOSAURUS:CVCL_1619]
- Phenotype : Cell Proliferation
- Library : Focused Ras Synthetic Lethal Human CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 2,389 | Full Dataset Size: 18,663
- Experimental Setup : Timecourse | Duration: 14 Doublings
- Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) < -1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The Wang et al., 2017 library contains the Wang et al., 2015 library + a sub-library targeting 497 additional protein-coding genes
Score Distribution
< 2.289
> -5.023