CRISPR Screen Dataset
Codina A (2019) - 1-PMID30797773
Title: Convergent Identification and Interrogation of Tumor-Intrinsic Factors that Modulate Cancer Immunity In Vivo.
Screen Details:
- Screen Rationale : Tumorigenicity
- Cell Type : Immortal Mouse Liver-derived Cell Line [CLO:0000537]
- Cell Line : IMC9 [CELLOSAURUS:CVCL_C8ZG]
- Phenotype : Tumorigenicity
- Library : Brie (mouse) [AddGene] | Type: CRISPRn | Format: in vivo | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 9 | Full Dataset Size: 19,590
- Experimental Setup : Implantation to Mouse Model | Duration: 90 Days | MOI: ~ 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- a p53-/-;Myc-primed mouse hepatocyte cell line was transfected with the Brie CRISPR library and implanted into a Nu/Nu mouse background. Genes whose knockout induced tumor formation based on the results of 3 separate analyses methods (RIGER, 3-way HitCaller and MAGECK) were considered high confidence screen hits
Score Distribution
< 0.99999
> 5.11e-16