CRISPR Screen Dataset
Tarumoto Y (2018) - 33-PMID29526696
Title: LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia.
Screen Details:
- Screen Rationale : Cell-essential genes from a targeted transcription factor screen
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : OCI-AML-3 [BTO:0004620, CELLOSAURUS:CVCL_1844]
- Phenotype : Cell Proliferation
- Library : CRISPRn Targeted Transcription Factor Domain Library (Vakoc, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 89 | Full Dataset Size: 1,427
- Experimental Setup : Timecourse | Duration: 12 Doublings | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) < -1.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- this is not a genome-wide screen; the authors screened a targeted transcription factor domain library in AML versus non-AML cells
Score Distribution
< 1.101997891
> -8.18138394