CRISPR Screen Dataset
Tarumoto Y (2018) - 2-PMID29526696
Title: LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia.
Screen Details:
- Screen Rationale : Cell-essential genes from a targeted transcription factor screen
- Cell Type : Acute Myeloid Leukemia Cell Line [BTO:0001883]
- Cell Line : MV4-11 [BTO:0006413, CELLOSAURUS:CVCL_0064]
- Phenotype : Cell Proliferation
- Library : Human Kinase Domain-Focused CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 38 | Full Dataset Size: 479
- Experimental Setup : Timecourse | Duration: 12 Doublings | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) < -1.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- this is not a genome-wide screen; the authors screened a targeted kinase domain library in AML versus non-AML cells
Score Distribution
< 1.070116404
> -7.439256018