CRISPR Screen Dataset

Viswanatha R (2018) - 2-PMID30051818

D. melanogaster

Title: Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality in cells.

Screen Rationale : Increased/decreased drug resistance
Cell Type : Drosophila Melanogaster Cell Line [EFO:0002935]
Cell Line : S2R+ [EFO:0005837, CELLOSAURUS:CVCL_Z831]
Phenotype : Response To Chemicals
Condition : Trametinib (50.0 nM) [ChEBI]
Library : CRISPRn Targeted Sub-libraries (Perrimon, 2018) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MAGeCK-MLE | Number of Hits: 91 | Full Dataset Size: 3,942
Experimental Setup : Drug Exposure | Duration: 30 Days
Significance : Authors have indicated that results with Score.1 (Z-score) < -10.0 OR Score.1 (Z-score) > 10.0 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Phenotypic readout: cell proliferation
the authors identified genes with whose loss conferred increased sensitivity to trametinib (negative Z-score) or increased resistance to trametinib (positive Z-score)

< 21.27

> -22.897