CRISPR Screen Dataset
Ju LG (2018) - 1-PMID29655804
Title: Characterization of WDR20: A new regulator of the ERAD machinery.
Screen Details:
- Screen Rationale : ERAD factors
- Cell Type : Embryonic Kidney Cell Line [BTO:0002733]
- Cell Line : HEK293T [BTO:0002181, CELLOSAURUS:CVCL_0063]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : None | Number of Hits: 1,138 | Full Dataset Size: 19,050
- Experimental Setup : Timecourse | Duration: 48 Hours
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- authors screened a TCRalpha-YFP-293T cell line using the GeckoV2 library to identify possible ERAD factors. TCRalpha is an established ERAD substrate, accumulation of TCRalpha suggests a loss of ERAD activity. The authors used FACS to isolate the top 1% of YFP expressing cells and sequenced sgRNAs from these cells to determine the relevant target genes.
Score Distribution
< 3698
> 52