Screen Summary

Patel SJ (2017) - 3-PMID28783722

Title: Identification of essential genes for cancer immunotherapy.

Screen Details:

Notes:
  • A two cell-type (2CT) CRISPR assay was carried out which consisted of human T cells as effectors and melanoma cells as targets in order to identify genes essential in tumours for the effector function of T cells (EFT). This 2CT-CRISPR assay identified loss-of-function genes in Mel624 melanoma cells conferring resistance to T cell-mediated cytolysis. 554 genes were identified at a false discovery rate (FDR) < 0.1%. (Re-analysis of 2CT-CRISPR enrichment screen with genes ranked by their second most enriched sgRNA.)

Score Distribution

< 554
> 1

CRISPR Screen Results (554 hits)