CRISPR Screen Dataset
Patel SJ (2017) - 3-PMID28783722
Title: Identification of essential genes for cancer immunotherapy.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : 624-mel [EFO:0006359, CELLOSAURUS:CVCL_8054]
- Phenotype : Cell Proliferation
- Condition : CD8+ T cells
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 554 | Full Dataset Size: 19,050
- Experimental Setup : T cell exposure | Duration: 12 Hours | MOI: < 0.3
- Significance : Authors have indicated that results with Score.2 (Second best guide score) > 0.5 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- A two cell-type (2CT) CRISPR assay was carried out which consisted of human T cells as effectors and melanoma cells as targets in order to identify genes essential in tumours for the effector function of T cells (EFT). This 2CT-CRISPR assay identified loss-of-function genes in Mel624 melanoma cells conferring resistance to T cell-mediated cytolysis. 554 genes were identified at a false discovery rate (FDR) < 0.1%. (Re-analysis of 2CT-CRISPR enrichment screen with genes ranked by their second most enriched sgRNA.)
Score Distribution
< 554
> 1