CRISPR Screen Dataset
Patel SJ (2017) - 2-PMID28783722
Title: Identification of essential genes for cancer immunotherapy.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : 624-mel [EFO:0006359, CELLOSAURUS:CVCL_8054]
- Phenotype : Cell Proliferation
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RIGER | Number of Hits: 508 | Full Dataset Size: 19,050
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: < 0.3
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Depletion analysis of Mel624 melanoma cells not exposed to T cells, i.e. no T cells day 7 versus no T cells day 17 cultures (post-transduction with the GeCKOv2 CRISPR library).
Score Distribution
< 90532.25
> 4.75