CRISPR Screen Dataset

Patel SJ (2017) - 1-PMID28783722

Title: Identification of essential genes for cancer immunotherapy.

Screen Details:

  • Screen Rationale : Cell-essential genes (melanoma immunotherapy resistance)
  • Screen Type : Positive Selection | Format: Pool
  • Cell Line : 624-mel | Type: Melanoma Cell Line
  • Throughput : High Throughput
  • Enzyme : CAS9
  • Phenotype : Viability
  • Experimental Setup : T cell exposure | Duration: 12 Hours | Condition Name: CD8+ T cells | MOI: < 0.3
  • Library : GECKO v2 | Type: CRISPRn | Methodology: Knockout
  • Analysis Method : RIGER | Number of Hits: 747 | Full Dataset Size: 19,050
  • Significance : Authors have indicated that results with Score.2 (p-Value) <= 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.
Notes:
  • A two cell-type (2CT) CRISPR assay was carried out which consisted of human T cells as effectors and melanoma cells as targets in order to identify genes essential in tumours for the effector function of T cells (EFT). This 2CT-CRISPR assay identified loss-of-function genes in Mel624 melanoma cells conferring resistance to T cell-mediated cytolysis. (Re-analysis of 2CT-CRISPR enrichment screen using RIGER.)

Score Distribution

< 89602.25
> 8.5

CRISPR Screen Results (747 hits)