CRISPR Screen Dataset

Reddy A (2017) - 12-PMID28985567

Title: Genetic and Functional Drivers of Diffuse Large B Cell Lymphoma.

Screen Details:

  • Screen Rationale : Cell-essential genes (lymphoma)
  • Screen Type : Positive Selection | Format: Pool
  • Cell Line : BJAB cell | Type: B-lymphoma Cell Line
  • Throughput : High Throughput
  • Enzyme : CAS9
  • Phenotype : Viability
  • Experimental Setup : Timecourse | Duration: 14 Doublings | MOI: ~ 0.4
  • Library : GECKO v2 | Type: CRISPRn | Methodology: Knockout
  • Analysis Method : Mann-Whitney U test | Number of Hits: 279 | Full Dataset Size: 18,862
  • Significance : Authors have indicated that results with Score.1 (CRISPR Score (CS)) > 0 AND Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.
Notes:
  • The authors screened lymphoma cell lines and determined which genes are significantly enriched after 14 doublings.

Score Distribution

< 2.889
> -4.233

CRISPR Screen Results (279 hits)