CRISPR Screen Dataset
Doench JG (2016) - 9-PMID26780180
Title: Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : A-375 [BTO:0002806, CELLOSAURUS:CVCL_0132]
- Phenotype : Cell Proliferation
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : STARS | Number of Hits: 528 | Full Dataset Size: 17,627
- Experimental Setup : Timecourse | Duration: 3 Weeks | MOI: < 1
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Negative selection screen with the GECKOv2 library screened in the lentiGuide vector (A-375 cells; STARS analysis at FDR < 10%).
Score Distribution
< 8.988086194
> 0.6734422