Screen Summary
Gilbert LA (2014) - 3-PMID25307932
Title: Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Screen Details:
- Screen Type : Negative Selection | Format: Pool
- Cell Line : K-562 cell | Type: Chronic Myeloid Leukemia Cell Line
- Throughput : High Throughput
- Enzyme : sunCas9
- Phenotype : Viability
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: < 1
- Library : CRISPRa (Weissman, 2014) | Type: CRISPRa | Methodology: Activation
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 142 | Full Dataset Size: 15,977
- Significance : Authors have indicated that results with Score.1 (Gamma (normalized log2e/t)) <= -0.1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- activation of hit genes results in decreased fitness
Score Distribution
< 0.190915104
> -0.523663585