CRISPR Screen Dataset
Yilmaz A (2018) - 1-PMID29662178
Title: Defining essential genes for human pluripotent stem cells by CRISPR-Cas9 screening in haploid cells.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Embryonic Stem Cell Line [BTO:0001581]
- Cell Line : h-pES10 [CELLOSAURUS:CVCL_AX68]
- Phenotype : Cell Proliferation
- Library : Human Activity-optimized genome-wide library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Kolmogorov-Smirnov | Number of Hits: 2,553 | Full Dataset Size: 18,166
- Experimental Setup : Timecourse | Duration: 23 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- Screen for essential genes (negative CRISPR score) and growth restrictive genes (positive CRISPR score) in a haploid human embryonic stem cell line
Score Distribution
< 4.58
> -3.48