CRISPR Screen Dataset
Goodwin JM (2017) - 4-PMID28877469
Title: Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.
Screen Details:
- Screen Rationale : Regulators of autophagy in the absence of FIP200
- Cell Type : Glioma Cell Line [BTO:0000711]
- Cell Line : H4 [BTO:0003704, CELLOSAURUS:CVCL_1239]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : CRISPRn Minipool (Murphy, 2017) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : DESeq2 | Number of Hits: 95 | Full Dataset Size: 1,120
- Experimental Setup : Timecourse | Duration: 7 Days
- Significance : Authors have indicated that results with Score.1 (Log2) > 1.0 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- targeted mini-pool screen for genes whose depletion results in increased p62 levels as determined by GFP-tag and FACS sorting in a FIP200 knock-out background
Score Distribution
< 4.325247097
> -3.958072135