Screen Summary
Goodwin JM (2017) - 4-PMID28877469
Title: Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.
Screen Details:
- Screen Type : Phenotype Screen | Format: Pool
- Cell Line : H4 neuroglioma cell | Type: Glioma Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Protein/peptide Accumulation
- Experimental Setup : Timecourse | Duration: 7 Days
- Library : CRISPRn Minipool (Murphy, 2017) | Type: CRISPRn | Methodology: Knockout
- Analysis Method : DESeq2 | Number of Hits: 95 | Full Dataset Size: 1,120
- Significance : Authors have indicated that results with Score.1 (Log2) > 1 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- targeted mini-pool screen for genes whose depletion results in increased p62 levels as determined by GFP-tag and FACS sorting in a FIP200 knock-out background
Score Distribution
< 4.325247097
> -3.958072135