CRISPR Screen Dataset
Goodwin JM (2017) - 1-PMID28877469
Title: Autophagy-Independent Lysosomal Targeting Regulated by ULK1/2-FIP200 and ATG9.
Screen Details:
- Screen Rationale : Regulators of NCOA4 abundance
- Cell Type : Glioma Cell Line [BTO:0000711]
- Cell Line : H4 [BTO:0003704, CELLOSAURUS:CVCL_1239]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : CRISPRn (Hoffman and Nyfeler, 2016) | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 1,718 | Full Dataset Size: 17,974
- Experimental Setup : Timecourse | Duration: 7 Days | MOI: = 0.5
- Significance : Authors have indicated that results with Score.2 (DESeq2) >= 0.5 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- knock-out of hit genes results in an accumulation of GFP-tagged NCOA4 in cells
Score Distribution
< 0
> -15.49