CRISPR Screen Dataset

Toledo CM (2015) - 2-PMID26673326

Title: Genome-wide CRISPR-Cas9 Screens Reveal Loss of Redundancy between PKMYT1 and WEE1 in Glioblastoma Stem-like Cells.

Screen Details:

  • Screen Rationale : Cell-essential genes (glioblastoma)
  • Screen Type : Negative Selection | Format: Pool
  • Cell Line : GSC-0827 | Type: Glioblastoma Cell Line
  • Throughput : High Throughput
  • Enzyme : CAS9
  • Phenotype : Viability
  • Experimental Setup : Timecourse | Duration: 23 Days | MOI: < 1
  • Library : GECKO | Type: CRISPRn | Methodology: Knockout
  • Analysis Method : BAGEL | Number of Hits: 1,243 | Full Dataset Size: 18,076
  • Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 3 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.
Notes:
  • This data set contains 3 ambiguous gene names and 75 unknown genes that were not loaded into ORCS.

Score Distribution

< 35.622
> -13.598

CRISPR Screen Results (1243 hits)