CRISPR Screen Dataset

Aguirre AJ (2016) - 17-PMID27260156

Title: Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting.

Screen Details:

  • The 33 cell line GeCKOv2 dataset from PMID:27260156 was re-analysed using the CERES method in PMID:29083409 and this CERES dataset was loaded into ORCS. To identify gene hits at an FDR < 0.05, ORCS estimated FDRs for the CERES GeCKOv2 dataset using customized gold-standard positive and negative sets of genes. The custom positive set included genes essential in at least 90% of cell lines in both the CERES Avana dataset (PMID:29083409) and the 10 cell lines included in Bertomeu et al. (PMID:29038160). The custom negative set included genes essential in zero cell lines from one of the two datasets and in <=10% of the other dataset. Genes scoring among the top 1,500 in >10% and at least two cell lines in either of the following three studies were further filtered from the negative set: PMID:27260156, PMID:27760321, PMID:28162770.

Score Distribution

< 2.2421543203004
> -2.18591237169281

CRISPR Screen Results (1870 hits)