CRISPR Screen Dataset
Aguirre AJ (2016) - 6-PMID27260156
Title: Genomic Copy Number Dictates a Gene-Independent Cell Response to CRISPR/Cas9 Targeting.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : COR-L105 [EFO:0002141, CELLOSAURUS:CVCL_1138]
- Phenotype : Cell Proliferation
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CERES | Number of Hits: 2,130 | Full Dataset Size: 17,627
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: < 1
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- The 33 cell line GeCKOv2 dataset from PMID:27260156 was re-analysed using the CERES method in PMID:29083409 and this CERES dataset was loaded into ORCS. To identify gene hits at an FDR < 0.05, ORCS estimated FDRs for the CERES GeCKOv2 dataset using customized gold-standard positive and negative sets of genes. The custom positive set included genes essential in at least 90% of cell lines in both the CERES Avana dataset (PMID:29083409) and the 10 cell lines included in Bertomeu et al. (PMID:29038160). The custom negative set included genes essential in zero cell lines from one of the two datasets and in <=10% of the other dataset. Genes scoring among the top 1,500 in >10% and at least two cell lines in either of the following three studies were further filtered from the negative set: PMID:27260156, PMID:27760321, PMID:28162770.
Score Distribution
< 2.01067619257712
> -2.10869589493529