CRISPR Screen Dataset
Reddy A (2017) - 5-PMID28985567
Title: Genetic and Functional Drivers of Diffuse Large B Cell Lymphoma.
Screen Details:
- Screen Rationale : Cell-essential genes and growth restrictive genes
- Cell Type : Diffuse Large B-cell Lymphoma Cell [BTO:0002521]
- Cell Line : HBL-1 [BTO:0002522, CELLOSAURUS:CVCL_4213]
- Phenotype : Cell Proliferation
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : Mann-Whitney U test | Number of Hits: 416 | Full Dataset Size: 18,862
- Experimental Setup : Timecourse | Duration: 14 Doublings | MOI: ~ 0.4
- Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors screened lymphoma cell lines and determined which genes are significantly enriched or depleted after 14 doublings.
Score Distribution
< 3.319
> -3.333