CRISPR Screen Dataset
Gilbert LA (2014) - 2-PMID25307932
Title: Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation.
Screen Details:
- Screen Rationale : Gene inhibition decreases fitness
- Cell Type : Chronic Myeloid Leukemia Cell Line [BTO:0002580]
- Cell Line : K-562 [BTO:0000664, CELLOSAURUS:CVCL_0004]
- Phenotype : Cell Proliferation
- Library : CRISPRi (Weissman, 2014) [AddGene] | Type: CRISPRi | Format: Pool | Enzyme: dCas9-KRAB | Methodology: Inhibition
- Analysis Method : Log2 Fold Change (L2FC) | Number of Hits: 1,345 | Full Dataset Size: 15,976
- Experimental Setup : Timecourse | Duration: 10 Days | MOI: < 1
- Significance : Authors have indicated that results with Score.1 (Gamma (normalized log2e/t)) <= -0.1 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- inhibition of hit genes results in decreased fitness
Score Distribution
< 0.150520629
> -0.698935214