CRISPR Screen Dataset
Lin DL (2017) - 1-PMID28720733
Title: Dengue Virus Hijacks a Noncanonical Oxidoreductase Function of a Cellular Oligosaccharyltransferase Complex.
Screen Details:
- Screen Rationale : Increased resistance to virus
- Cell Type : Hepatoma Cell Line [BTO:0000578]
- Cell Line : HuH-7 [BTO:0001950, CELLOSAURUS:CVCL_0336]
- Phenotype : Response To Virus
- Condition : Virus: Dengue virus 2 Thailand/16681/84 [NCBI Taxid 31634]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 24 | Full Dataset Size: 19,050
- Experimental Setup : Virus Exposure | Duration: 14 Days | MOI: = 0.3
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Phenotypic readout: cell proliferation
- screen to identify host proteins necessary for DENV-mediated cell death in Huh7.5.1 human hepatoma cell.
Score Distribution
< 0.768196
> 0.00045