CRISPR Screen Dataset
Stewart SE (2017) - 1-PMID28775154
Title: A genome-wide CRISPR screen reconciles the role of N-linked glycosylation in galectin-3 transport to the cell surface.
Screen Details:
- Screen Rationale : Regulators or GAL3 surface localization
- Cell Type : Cervical Adenocarcinoma Cell Line [BTO:0005590]
- Cell Line : HeLa [BTO:0000567, CELLOSAURUS:CVCL_0030]
- Phenotype : Protein/peptide Distribution [APO:0000209]
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : RSA | Number of Hits: 1,174 | Full Dataset Size: 20,916
- Experimental Setup : Timecourse | Duration: 21 Days | MOI: ~ 0.2
- Significance : Authors have indicated that results with Score.1 (Log10 (p-value)) > 1.3 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Authors screened for genes required for cell surface localization of Gal-3.
Score Distribution
< 12.151
> -1