CRISPR Screen Dataset

Meyers RM (2017) - 176-PMID29083409

Title: Computational correction of copy number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells.

Screen Details:

  • Screen Rationale : Cell-essential genes
  • Cell Type : MDA-MB-435 Cell [BTO:0001567]
  • Cell Line : MDA-MB-435S [BTO:0003941]
  • Phenotype : Cell Proliferation
  • Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
  • Analysis Method : CERES | Number of Hits: 2,081 | Full Dataset Size: 17,670
  • Experimental Setup : Timecourse | Duration: 21 Days | MOI: < 1
  • Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.
Notes:
  • To identify gene hits at an FDR < 0.05, ORCS estimated FDRs for the CERES Avana dataset using customized gold-standard positive and negative sets of genes. The custom positive set included genes essential in at least 90% of cell lines in both the CERES Avana dataset (PMID:29083409) and the 10 lines included in Bertomeu et al. (PMID:29038160). The custom negative set included genes essential in zero cell lines from one of the two datasets and in <=10% of the other dataset.

Score Distribution

< 2.02272265836
> -1.7273057755

CRISPR Screen Results (2081 hits)