CRISPR Screen Dataset
Breslow DK (2018) - 2-PMID29459677
Title: A CRISPR-based screen for Hedgehog signaling provides insights into ciliary function and ciliopathies.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Embryonic Fibroblast Cell Line [BTO:0001958]
- Cell Line : NIH 3T3 [BTO:0000944, CELLOSAURUS:CVCL_0594]
- Phenotype : Cell Proliferation
- Library : Bassik Mouse CRISPR Knockout Library [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : CasTLE | Number of Hits: 1,516 | Full Dataset Size: 22,414
- Experimental Setup : Timecourse | Duration: 15 Days | MOI: ~ 0.3
- Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by '' in the HIT column of the table below.
Notes:
- Genes whose depletion caused a growth defect were determined so that they could be excluded from Hedgehog pathway specific analyses.
Score Distribution
< 256
> 0