CRISPR Screen Dataset
Hart T (2015) - 5-PMID26627737
Title: High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.
Screen Details:
- Screen Rationale : Cell-essential genes
- Cell Type : Colonic Adenocarcinoma Cell Line [BTO:0001913]
- Cell Line : DLD-1 [BTO:0000391, CELLOSAURUS:CVCL_0248]
- Phenotype : Cell Proliferation
- Library : TKO (Toronto Knockout) v1 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 1,893 | Full Dataset Size: 17,219
- Experimental Setup : Timecourse | Duration: 18 Days | MOI: ~ 0.3
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 3.57 are to be considered significant. These results are indicated by ' ' in the HIT column of the table below.
Notes:
- Genes with a Bayes Factor (BF) above the threshold of 3.57 at an FDR of 5% (FDR < 0.05) were identified as fitness genes (hits) for this cell line in this CRISPR screen.
Score Distribution
< 60.8
> -25.142