CRISPR Screen Dataset
Hart T (2015) - 4-PMID26627737
Title: High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.
Screen Details:
- Screen Rationale : Cell-essential genes (retinal pigment epithelium)
- Screen Type : Negative Selection | Format: Pool
- Cell Line : hTERT-RPE1 cell | Type: Retinal Pigment Epithelium Cell Line
- Throughput : High Throughput
- Enzyme : CAS9
- Phenotype : Viability
- Experimental Setup : Timecourse | Duration: 18 Days | MOI: ~ 0.3
- Library : TKO (Toronto Knockout) v1 | Type: CRISPRn | Methodology: Knockout
- Analysis Method : BAGEL | Number of Hits: 2,038 | Full Dataset Size: 17,212
- Significance : Authors have indicated that results with Score.1 (Bayes Factor) > 6.84 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- Genes with a Bayes Factor (BF) above the threshold of 6.84 at an FDR of 5% (FDR < 0.05) were identified as fitness genes (hits) for this cell line in this CRISPR screen.
Score Distribution
< 152.816
> -63.793