CRISPR Screen Dataset

Meyers RM (2017) - 85-PMID29083409

H. sapiens

Title: Computational correction of copy number effect improves specificity of CRISPR-Cas9 essentiality screens in cancer cells.

Screen Rationale : Cell-essential genes
Cell Type : Oral Squamous Cell Carcinoma Cell Line [BTO:0002211]
Cell Line : HSC-3 [BTO:0002025, CELLOSAURUS:CVCL_1288]
Phenotype : Cell Proliferation
Library : Avana | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : CERES | Number of Hits: 1,479 | Full Dataset Size: 17,670
Experimental Setup : Timecourse | Duration: 21 Days | MOI: < 1
Significance : Authors have indicated that results with Score.2 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

To identify gene hits at an FDR < 0.05, ORCS estimated FDRs for the CERES Avana dataset using customized gold-standard positive and negative sets of genes. The custom positive set included genes essential in at least 90% of cell lines in both the CERES Avana dataset (PMID:29083409) and the 10 lines included in Bertomeu et al. (PMID:29038160). The custom negative set included genes essential in zero cell lines from one of the two datasets and in <=10% of the other dataset.

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