CRISPR Screen Dataset
Konermann S (2015) - 2-PMID25494202
Title: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Screen Details:
- Screen Rationale : Gene activation increases drug resistance
- Cell Type : Melanoma Cell Line [BTO:0000849]
- Cell Line : A-375 [BTO:0002806, CELLOSAURUS:CVCL_0132]
- Phenotype : Response To Chemicals
- Condition : PLX-4720 (2.0 µM) [ChEBI]
- Library : SAM v1 (Puromycin) [AddGene] | Type: CRISPRa | Format: Pool | Enzyme: SAM (NLS-dCas9-VP64/MS2-p65-HSF1) | Methodology: Activation
- Analysis Method : RIGER | Number of Hits: 100 | Full Dataset Size: 23,430
- Experimental Setup : Drug Exposure | Duration: 21 Days | MOI: < 0.2
- Significance : Authors have indicated that ALL results are to be considered significant.
Notes:
- Phenotypic readout: cell proliferation
Score Distribution
< 1.8175
> 1.5831