CRISPR Screen Dataset
Konermann S (2015) - 2-PMID25494202
Title: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Screen Details:
- Screen Rationale : Gene activation increases drug resistance (melanoma to PLX-4720 (BRAF inhibitor))
- Screen Type : Positive Selection | Format: Pool
- Cell Line : A-375 cell | Type: Melanoma Cell Line
- Throughput : High Throughput
- Enzyme : SAM (NLS-dCas9-VP64/MS2-p65-HSF1)
- Phenotype : Resistance To Chemicals
- Experimental Setup : Drug Exposure | Duration: 21 Days | Condition Name: PLX-4720 (2 µM) | MOI: < 0.2
- Library : SAM v1 (Puromycin) | Type: CRISPRa | Methodology: Activation
- Analysis Method : RIGER | Number of Hits: 100 | Full Dataset Size: 23,430
- Significance : Authors have indicated that ALL results are to be considered significant.
Score Distribution
< 1.8175
> 1.5831