CRISPR Screen Dataset

Lin L (2023) - 3-PMID37883554

H. sapiens

Title: Unbiased transcription factor CRISPR screen identifies ZNF800 as master repressor of enteroendocrine differentiation.

Screen Rationale : Regulation of enteroendocrine cell (EEC) differentiation
Cell Type : Intestinal Organoid Model
Cell Line : Organoid: primary cell derived small intestinal organoid line
Phenotype : Protein/peptide Accumulation [APO:0000149]
Library : Brunello TF targeted library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : Differential MAGeCK Beta-scores | Number of Hits: 30 | Full Dataset Size: 1,800
Experimental Setup : Timecourse | Duration: 12 Days | MOI: < 0.2
Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by 'Yes' in the HIT column of the table below.

Hit genes with a larger CHGA+ EEC beta score (column 1) than MUC2+ Goblet beta score (column 3) have enriched sgRNAs in an EEC population compared to goblet cell population, suggesting KO of the hit results in increased EEC numbers and the gene normally represses EEC differentiation.
Hit genes with a larger MUC2+ Goblet beta score (column 3) than CHGA+ EEC beta score (column 1) have depleted sgRNAs in an EEC population compared to goblet cell population, suggesting KO of the hit results in decreased EEC numbers and the gene normally promotes EEC differentiation.

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