CRISPR Screen Dataset

Lin L (2023) - 2-PMID37883554

H. sapiens

Title: Unbiased transcription factor CRISPR screen identifies ZNF800 as master repressor of enteroendocrine differentiation.

Screen Rationale : Cell-essential and growth restrictive genes in differentiated cells
Cell Type : Intestinal Organoid Model
Cell Line : Organoid: primary cell derived small intestinal organoid line
Phenotype : Cell Proliferation
Library : Brunello TF targeted library | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
Analysis Method : MAGeCK-MLE | Number of Hits: 315 | Full Dataset Size: 1,800
Experimental Setup : Timecourse | Duration: 12 Days | MOI: < 0.2
Significance : Authors have indicated that results with Score.1 (Beta Score) < -1.0 AND Score.3 (FDR) < 0.05 OR Score.1 (Beta Score) > 1.0 AND Score.3 (FDR) < 0.05 are to be considered significant. These results are indicated by 'Yes' in the HIT column of the table below.

Hit genes with a significant negative beta score have depleted sgRNA counts in a differentiated cell sample suggesting the gene is essential for differentiated cell survival
Hit genes with a significant positive beta score have enriched sgRNA counts in a differentiated cell sample suggesting KO of the gene increases differentiated cell survival

< 3.5055

> -3.5194