CRISPR Screen Dataset

Lo YH (2025) - 3-PMID40813572

H. sapiens

Title: Large-scale CRISPR screening in primary human 3D gastric organoids enables comprehensive dissection of gene-drug interactions.

Screen Rationale : Increased/decreased organoid growth upon inhibition
Cell Type : Gastric Tumor Organoid Model
Cell Line : Organoid: TP53/APC knockout gastric tumor organoid line
Phenotype : Cell Proliferation
Library : CRISPRi targeted library (Chen, 2025) | Type: CRISPRi | Format: Pool | Enzyme: Cas9 | Methodology: Inhibition
Analysis Method : Mann-Whitney U test | Number of Hits: 396 | Full Dataset Size: 1,952
Experimental Setup : Timecourse | Duration: 7 Doublings | MOI: = 0.3
Significance : Authors used a complex method to determine significant results. Please refer to the source publication for more details. Significant results are indicated by 'Yes' in the HIT column of the table below.

A targeted CRISPRi screen was carried out in a human Tp53/APC double knockout gastric tumor organoid model. The CRISPRi library targeted 1952 genes encoding DNA and RNA-binding proteins in the organoids. To call gene hits, a "screen score" was defined as | gamma z-score from negative control gene distribution | x -log10 p-value.

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