CRISPR Screen Dataset
He B (2022) - 1-PMID36476853
Title: Mitochondrial cristae architecture protects against mtDNA release and inflammation.
Screen Details:
- Screen Rationale : Regulation of the IFN-I response
- Cell Type : Embryonic Fibroblast Cell Line [BTO:0001958]
- Cell Line : MEF [BTO:0002572]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Library : MitoCarta2.0 | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 59 | Full Dataset Size: 1,146
- Experimental Setup : Timecourse | Duration: 7 Days | MOI: = 0.3
- Significance : Authors have indicated that results with Score.1 (Log2FC) > 0.58 AND Score.2 (p-Value) < 0.05 OR Score.1 (Log2FC) < -0.58 AND Score.2 (p-Value) < 0.05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- KO of genes with a significant positive log2foldchange induced expression of a fluorescent protein reporter in place of the ISG15 coding sequence, suggesting that KO of the gene activates the IFN-I response
- KO of genes with a significant negative log2foldchange inhibited expression of a fluorescent protein reporter in place of the ISG15 coding sequence, suggesting that KO of the gene inhibits the IFN-I response
Score Distribution
< 1.7158
> -1.0921