CRISPR Screen Dataset
Ngo AM (2023) - 1-PMID36971544
Title: Genome-Scale Analysis of Cellular Restriction Factors That Inhibit Transgene Expression from Adeno-Associated Virus Vectors.
Screen Details:
- Screen Rationale : Restriction factors of rAAV transgene expression
- Cell Type : Lung Cancer Cell Line [BTO:0000762, EFO:0002934]
- Cell Line : A-549 [BTO:0000018, CELLOSAURUS:CVCL_0023]
- Phenotype : Protein/peptide Accumulation [APO:0000149]
- Condition : Transduction with rAAV2-GFP (10000.0 vg/cell (viral genomes/cell))
- Library : GECKO v2 [AddGene] | Type: CRISPRn | Format: Pool | Enzyme: Cas9 | Methodology: Knockout
- Analysis Method : MaGeCK | Number of Hits: 20 | Full Dataset Size: 20,721
- Experimental Setup : Other | Duration: 20 Days | MOI: = 0.4
- Significance : Authors have indicated that results with Score.1 (MaGeCK Score) < 1e-05 are to be considered significant. These results are indicated by '' in the HIT column of the table below.
Notes:
- The authors used a KO library to screen for genes whose loss results in increased expression of rAAV2-GFP, suggesting the gene normally functions in restricting rAAV transgene expression
Score Distribution
< 1
> 1.24e-24